The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001112690.1 | NP_001106160.1 | 5 - 5 | 845 | 91 |
| XM_021066031.1 | XP_020921690.1 | - | 788 | 91 | |
| XM_021066032.1 | XP_020921691.1 | - | 1394 | 91 | |
| GenBank mRNA | EF591082.1 | - | 5 - 5 | 845 | 91 |