The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001178126.1 | NP_001171597.1 | 3 - 3 | 203 | |
| NM_001256296.1 | NP_001243225.1 | 2 - 2 | 490 | 84 | |
| GenBank mRNA | AJ318021.1 | - | 3 - 3 | 206 | |
| AJ318022.1 | - | 3 - 3 | 203 | ||
| AJ319670.1 | - | 2 - 2 | 441 | 84 | |
| BG756003.1 | - | 3 - 3 | 203 | ||