The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | XM_022425584.1 | XP_022281292.1 | 3 - 3 | 460 | 147 |
| XM_533666.6 | XP_533666.2 | 4 - 4 | 545 | 147 | |