Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: E2f1
Entrez Gene ID: 42550
Gene Name: E2F transcription factor 1
Gene Aliases: CG6376, DRTF1/E2F, DmE2F-1, Dmel\CG6376, Dmel_CG6376, Dp, E(Sev-CycE)3A, E(var)3-93E, E(var)3-95E, E(var)93E, E2F, E2F-1, E2F1, E2f, Evar(3)164, dE2F, dE2F1, dE2f, dE2f1, de2f1, def21, drosE2F1, e2f, e2f1, l(3)07172, l(3)j3B1, l(3)j3C2, l(3)rM729
Chromosome Location: Chr.3R: 21620304 - 21660871 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0011766

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001275880.2	NP_001262809.1	2 - 3	1388	84
	NM_001300525.1	NP_001287454.1	2 - 3	977	84
	NM_001300526.1	NP_001287455.1	4 - 5	1444	84
	NM_079713.3	NP_524437.2	2 - 3	1768	84
	NM_169961.3	NP_732646.1	4 - 5	1005	84
	NM_169962.3	NP_732647.1	2 - 3	1569	84
GenBank mRNA	BT016096.1	-	4 - 5	978	84