The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001142343.1 | NP_001135815.1 | 1 - 1 | 266 | 75 |
| NM_004072.2 | NP_004063.1 | 1 - 1 | 266 | 75 | |
| GenBank mRNA | BC106927.2 | - | 1 - 1 | 80 | 75 |
| BC106928.1 | - | 1 - 1 | 80 | 75 | |
| U79527.1 | - | 1 - 1 | 272 | 75 | |