Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: FER
Entrez Gene ID: 2241
Gene Name: FER tyrosine kinase
Gene Aliases: PPP1R74, TYK3, p94-Fer
Chromosome Location: Chr.5: 108746085 - 109196841 on Build GRCh38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001308028.1	NP_001294957.1	18 - 18	7240	86
	NM_001308031.1	NP_001294960.1	11 - 11	6190	86
	NM_005246.3	NP_005237.2	20 - 20	7626	86
	XM_011543276.2	XP_011541578.1	12 - 12	6442	86
GenBank mRNA	AK123847.1	-	1 - 1	855	86

Non Gene Transcripts

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	IMAGE Clone ID	Transcript Location	Amplicon Length
GenBankmRNA	DA789467.1	-	1	35		Chr. 5 - 109192314 - 109192891 [+] on Build GRCh38	86
GenBankmRNA	DA121195.1	-	1	161		Chr. 5 - 109192188 - 109192719 [+] on Build GRCh38	86
GenBankmRNA	DA318579.1	-	1	135		Chr. 5 - 109192214 - 109192782 [+] on Build GRCh38	86
GenBankmRNA	BX952769.1	-	1	41		Chr. 5 - 109192308 - 109192595 [+] on Build GRCh38	86
GenBankmRNA	DA800183.1	-	1	468		Chr. 5 - 109191881 - 109192433 [+] on Build GRCh38	86

Adjacent Gene Details

	Gene Name	Gene Symbol	Alias	Distance	Relative Direction	Location Chromosome
Downstream	uncharacterized LOC285638	LOC285638		42.1kb	Anti-sense	Chr. - 109319454 - 109330456 on Build GRCh38

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