The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_000181.3 | NP_000172.2 | 3 - 3 | 699 | 63 |
| NR_120531.1 | 3 - 3 | 699 | 63 | ||
| XM_005250297.3 | XP_005250354.1 | 3 - 3 | 694 | 63 | |
| XM_011516114.1 | XP_011514416.1 | 2 - 2 | 358 | 63 | |
| XM_017012093.1 | XP_016867582.1 | 2 - 2 | 358 | 63 | |
| GenBank mRNA | AK223406.1 | - | 3 - 3 | 598 | 63 |
| BC014142.2 | - | 3 - 3 | 569 | 63 | |
| CD014094.1 | - | 3 - 3 | 581 | 63 | |
| M15182.1 | - | 3 - 3 | 594 | 63 | |