The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_002127.5 | NP_002118.1 | 1 - 1 | 50 | 85 |
| GenBank mRNA | AK302054.1 | - | 1 - 1 | 50 | 85 |
| DC397107.1 | - | 1 - 1 | 50 | 85 | |