The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_006120.3 | NP_006111.2 | 2 - 2 | 432 | 93 |
| GenBank mRNA | AK291858.1 | - | 2 - 2 | 368 | 93 |
| BC011447.1 | - | 2 - 2 | 405 | 93 | |
| BC026279.1 | - | 2 - 2 | 361 | 93 | |
| CR450324.1 | - | 2 - 2 | 346 | 93 | |
| X62744.1 | - | 2 - 2 | 391 | 93 | |