The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_001304466.1 | NP_001291395.1 | 2 - 2 | 296 | 80 |
GenBank mRNA | AX775997.1 | - | 2 - 2 | 145 | 80 |
U81375.1 | - | 2 - 2 | 145 | 80 |