The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001178098.1 | NP_001171569.1 | 7 - 7 | 1086 | 81 |
| NM_001770.5 | NP_001761.3 | 7 - 7 | 1086 | 81 | |
| XM_006721103.3 | XP_006721166.1 | 6 - 6 | 1023 | 81 | |
| XM_017023893.1 | XP_016879382.1 | 6 - 6 | 887 | 81 | |
| GenBank mRNA | AK130657.1 | - | 6 - 6 | 472 | 81 |
| AK301167.1 | - | 7 - 7 | 1004 | 81 | |
| AK304456.1 | - | 8 - 8 | 871 | 81 | |
| AK313577.1 | - | 7 - 7 | 1057 | 81 | |
| BC006338.2 | - | 7 - 7 | 1068 | 81 | |
| M21097.1 | - | 7 - 7 | 1040 | 81 | |
| X13312.1 | - | 7 - 7 | 1039 | 81 | |