The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_213841.2 | NP_999006.1 | 3 - 3 | 251 | 124 |
| XM_013985798.2 | XP_013841252.1 | - | 538 | 124 | |
| GenBank mRNA | AF034646.1 | - | 3 - 3 | 205 | 124 |
| AJ000221.1 | - | 3 - 3 | 251 | 124 | |