The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | XM_005639887.3 | XP_005639944.1 | 7 - 7 | 1169 | 98 |
| XM_005639888.3 | XP_005639945.1 | 8 - 8 | 1324 | 98 | |
| XM_022414322.1 | XP_022270030.1 | 8 - 8 | 1409 | 98 | |
| XM_022414323.1 | XP_022270031.1 | 9 - 9 | 1503 | 98 | |
| XM_535853.6 | XP_535853.3 | 7 - 7 | 1227 | 98 | |