The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001252264.1 | NP_001239193.1 | 1 - 1 | 349 | 113 |
| XM_005632022.3 | XP_005632079.1 | 1 - 1 | 345 | 113 | |
| XM_022406218.1 | XP_022261926.1 | 1 - 1 | 362 | 113 | |
| GenBank mRNA | DN371621.1 | - | - | 304 | 113 |