The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | XM_005637517.2 | XP_005637574.1 | 6 - 6 | 1200 | 119 |
| XM_005637518.2 | XP_005637575.1 | 7 - 7 | 1484 | 119 | |
| XM_005637520.2 | XP_005637577.1 | 6 - 6 | 1484 | 119 | |
| XM_005637521.2 | XP_005637578.1 | 6 - 6 | 1452 | 119 | |
| XM_005637522.3 | XP_005637579.1 | 6 - 6 | 1200 | 119 | |
| XM_005637523.3 | XP_005637580.1 | 6 - 6 | 1401 | 119 | |
| XM_005637524.3 | XP_005637581.1 | 6 - 6 | 1270 | 119 | |
| XM_005637525.2 | XP_005637582.1 | 5 - 5 | 1056 | 119 | |
| XM_005637526.2 | XP_005637583.1 | 6 - 6 | 1200 | 119 | |