The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001024633.1 | NP_001019804.1 | 3 - 3 | 561 | 165 |
| GenBank mRNA | AY832859.1 | - | 3 - 3 | 561 | 165 |