The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001287170.1 | NP_001274099.1 | 3 - 3 | 685 | 151 |
| XM_022421759.1 | XP_022277467.1 | 3 - 3 | 529 | 151 | |
| XM_022421760.1 | XP_022277468.1 | 5 - 5 | 850 | 151 | |
| XM_022421761.1 | XP_022277469.1 | 5 - 5 | 786 | 151 | |
| XM_022421762.1 | XP_022277470.1 | 6 - 6 | 984 | 151 | |
| XM_022421763.1 | XP_022277471.1 | 5 - 5 | 773 | 151 | |
| XM_022421764.1 | XP_022277472.1 | 7 - 7 | 1023 | 151 | |