The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_001097981.1 | NP_001091450.1 | 3 - 3 | 656 | 116 |
GenBank mRNA | EF450456.1 | - | 3 - 3 | 656 | 116 |