The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001204842.1 | NP_001191771.1 | 2 - 2 | 2118 | 97 |
| XM_005625666.2 | XP_005625723.1 | 4 - 4 | 2356 | 97 | |
| XM_005625667.3 | XP_005625724.1 | 3 - 3 | 2234 | 97 | |