The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | XM_005639548.3 | XP_005639605.1 | 4 - 4 | 2467 | 156 |
| XM_022414009.1 | XP_022269717.1 | 5 - 5 | 2150 | 156 | |
| XM_022414010.1 | XP_022269718.1 | 4 - 4 | 2201 | 156 | |