Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: shot
Entrez Gene ID: 36542
Gene Name: short stop
Gene Aliases: 42/4, CG18076, CG18637, Dmel\CG18076, Dmel_CG18076, Grv, Kak, Shot, Su(Mir)1, grv, kak, kakp, kop, l(2)CA4, l(2)k03010, l(2)k04204, l(2)k05434, l(2)k05821, l(2)k10821, l(2)k15606, shortstop/kakapo
Chromosome Location: Chr.2R: 13864237 - 13942110 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0013733

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001144185.2	NP_001137657.1	7 - 8	2115	82
	NM_001144186.2	NP_001137658.1	7 - 8	2115	82
	NM_001144187.2	NP_001137659.1	7 - 8	2115	82
	NM_001144188.2	NP_001137660.1	7 - 8	2338	82
	NM_001144189.2	NP_001137661.1	7 - 8	2338	82
	NM_001169676.1	NP_001163147.1	4 - 5	2131	82
	NM_001169677.1	NP_001163148.1	7 - 8	2115	82
	NM_001169678.1	NP_001163149.1	7 - 8	2338	82
	NM_001169679.2	NP_001163150.1	4 - 5	2131	82
	NM_001259378.1	NP_001246307.1	4 - 5	2131	82
	NM_001259379.1	NP_001246308.1	4 - 5	2131	82
	NM_001259380.1	NP_001246309.1	8 - 9	2133	82
	NM_001259381.1	NP_001246310.1	8 - 9	2175	82
	NM_001259383.1	NP_001246312.1	4 - 5	2131	82
	NM_001259384.1	NP_001246313.1	4 - 5	2131	82
	NM_079009.3	NP_523733.2	7 - 8	2338	82
	NM_166015.2	NP_725335.1	7 - 8	2115	82
	NM_166016.2	NP_725336.1	7 - 8	2115	82
	NM_166017.2	NP_725337.1	7 - 8	2338	82
	NM_166018.3	NP_725338.1	4 - 5	2131	82
	NM_166019.3	NP_725339.1	4 - 5	2131	82