Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Trf4-1
Entrez Gene ID: 31795
Gene Name: Topoisomerase related function 4-1
Gene Aliases: AAF46390, CG11265, DmTRF4-1, Dmel\CG11265, Dmel_CG11265, Trf4, dTrf4-1
Chromosome Location: Chr.X: 8560322 - 8570159 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0030049

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001103443.2	NP_001096913.1	4 - 5	1984	77
	NM_001103444.2	NP_001096914.1	4 - 5	1971	77
	NM_001272411.1	NP_001259340.1	3 - 4	2276	77
	NM_132262.3	NP_572490.2	3 - 4	2241	77
	NM_206658.4	NP_996381.1	3 - 4	2403	77
	NM_206659.3	NP_996382.2	3 - 4	2208	77
	NM_206660.2	NP_996383.2	3 - 4	1978	77
GenBank mRNA	BT010282.1	-	3 - 4	2090	77