Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Sema1a
Entrez Gene ID: 34192
Gene Name: Semaphorin 1a
Gene Aliases: BcDNA:RE36155, CG18405, CT26404, D-Sema 1a, D-Sema I, D-Sema-I, D-SemaI, D-semaI, Dmel\CG18405, Dmel_CG18405, Dsema 1a, Dsema-I, Fas-IV, FasIV, SEMA-1A, Sema 1a, Sema-1a, Sema-I, Sema1A, SemaI, d-sema I, d-semaI, l(2)k13702, sema, sema 1a, sema-1a, sema-I, sema1a
Chromosome Location: Chr.2L: 8542147 - 8672041 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0011259

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001201802.2	NP_001188731.1	12 - 13	2461	69
	NM_001201803.2	NP_001188732.1	11 - 12	2575	69
	NM_001273336.1	NP_001260265.1	11 - 12	2575	69
	NM_001273337.1	NP_001260266.1	11 - 12	2534	69
	NM_001273338.1	NP_001260267.1	11 - 12	2575	69
	NM_135399.5	NP_609243.3	11 - 12	2255	69
	NM_164830.5	NP_723407.2	11 - 12	2575	69