Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: crol
Entrez Gene ID: 34592
Gene Name: crooked legs
Gene Aliases: CG14938, Crol, Dmel\CG14938, Dmel_CG14938, ORE-15, anon-EST:Liang-1.74, anon-EST:Liang-2.48, clone 1.74, clone 2.48, croI, crol alpha, crol beta, crol gamma, l(2)04418, l(2)s2346
Chromosome Location: Chr.2L: 11794327 - 11809438 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0020309

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001259064.2	NP_001245993.1	6 - 7	3578	55
	NM_001273461.1	NP_001260390.1	7 - 8	2767	55
	NM_001273462.1	NP_001260391.1	5 - 6	2680	55
	NM_001273464.1	NP_001260393.1	7 - 8	3042	55
	NM_057895.4	NP_477243.1	7 - 8	3688	55
	NM_057896.4	NP_477244.1	7 - 8	3070	55
	NM_164971.2	NP_723687.1	7 - 8	2790	55
GenBank mRNA	AF020347.1	-	7 - 8	3688	55
	AF020349.1	-	7 - 8	3070	55
	AY119553.1	-	4 - 5	2108	55