Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Pfk
Entrez Gene ID: 36060
Gene Name: Phosphofructokinase
Gene Aliases: BcDNA.GH12192, BcDNA:GH12192, CG4001, Dmel\CG4001, Dmel_CG4001, PFK, PFK1, l(2)06339, pfk
Chromosome Location: Chr.2R: 10109740 - 10117457 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0003071

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001259305.1	NP_001246234.1	9 - 10	1532	67
	NM_001273918.1	NP_001260847.1	8 - 9	1448	67
	NM_078952.3	NP_523676.1	9 - 10	1532	67
	NM_165746.3	NP_724890.1	5 - 6	1503	67
	NM_165747.4	NP_724891.2	5 - 6	1503	67
GenBank mRNA	AF145673.1	-	9 - 10	1532	67
	BT099630.1	-	5 - 6	1173	67
	BT133189.1	-	5 - 6	1096	67