Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Pkc53E
Entrez Gene ID: 48311
Gene Name: Protein C kinase 53E
Gene Aliases: CG6622, DPKC, Dmel\CG6622, Dmel_CG6622, Dpkc1, PK-C, PKC, PKC 53B, PKC 53E, PKC-53B, PKC-53E, PKC1, PKC53E, PKC53e, Pkc1, dPKC, dPKC53E(br), pck53E, pkc53e
Chromosome Location: Chr.2R: 16924655 - 16945126 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0003091

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001103880.3	NP_001097350.1	14 - 15	2262	76
	NM_001259448.1	NP_001246377.1	9 - 10	1593	76
	NM_001259449.1	NP_001246378.1	14 - 15	2202	76
	NM_001274106.1	NP_001261035.1	13 - 14	2261	76
	NM_057334.3	NP_476682.1	13 - 14	2744	76
	NM_166201.3	NP_725626.1	14 - 15	2265	76
GenBank mRNA	AY095003.1	-	14 - 15	2236	76
GenBank mRNA	BT120171.1	-	9 - 10	1593	76