Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: RhoGEF3
Entrez Gene ID: 38050
Gene Name: Rho guanine nucleotide exchange factor 3
Gene Aliases: CG1225, CG13881, CG13882, CG13883, CG42377, CG42378, CG43976, Dmel\CG43976, Dmel_CG43976, DrhoGEF3, RhoGEF, rhoGEF3
Chromosome Location: Chr.3L: 277437 - 305292 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0264707

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001144390.2	NP_001137862.1	6 - 7	1998	59
	NM_001144391.1	NP_001137863.1	4 - 5	1911	59
	NM_001169836.1	NP_001163307.1	6 - 7	1735	59
	NM_001169837.1	NP_001163308.1	6 - 7	1810	59
	NM_001274273.1	NP_001261202.1	15 - 16	9764	59
	NM_001382119.1	NP_001368935.1	5 - 6	1862	59
	NM_138180.4	NP_612024.4	15 - 16	9767	59
	NM_138182.2	NP_612026.2	6 - 7	2154	59
	NM_138183.3	NP_612027.2	6 - 7	1998	59
	NM_167823.2	NP_728515.1	6 - 7	1827	59
	NM_167824.2	NP_728516.1	6 - 7	1821	59
	NM_167825.2	NP_728517.1	5 - 6	1806	59
GenBank mRNA	BT011402.1	-	6 - 7	1827	59
	BT016166.1	-	6 - 7	1780	59
	BT044233.1	-	6 - 7	1978	59
	BT099707.1	-	6 - 7	2154	59