Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: eIF4E1
Entrez Gene ID: 45525
Gene Name: eukaryotic translation initiation factor 4E1
Gene Aliases: 0260/09, 0587/11, 0589/11, 0919/12, 1004/13, 2, 7238, CBP, CG4035, CT13384, CT39424, CT39426, D-eIF4E, Dmel\CG4035, Dmel_CG4035, EIF4E, Eif4E, Eif4e, IF4E, dEif-4e, dEif4e, deIF-4E, deIF4E, eIF 4E, eIF-4E, eIF-4E1, eIF-4E2, eIF-4EII, eIF-4e, eIF4E, eIF4E-1, eIF4E-1/2, eIF4E-2, eIF4EI, eIF4e, eif-4E, eif-4e, eif4e, elF4E, l(3)07238, l(3)67Af, l(3)S025007, l(3)S026009, l(3)S058711, l(3)S091912
Chromosome Location: Chr.3L: 9399618 - 9402460 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0015218

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001274697.1	NP_001261626.1	6 - 7	1081	60
	NM_001274698.1	NP_001261627.1	5 - 6	1382	60
	NM_080090.3	NP_524829.1	5 - 6	1073	60
	NM_168333.2	NP_729480.1	4 - 5	745	60
	NM_168334.1	NP_729481.1	5 - 6	1019	60
	NM_168335.2	NP_729482.1	5 - 6	1312	60
	NM_168336.2	NP_729483.1	5 - 6	1113	60
	NM_168337.2	NP_729484.1	5 - 6	1041	60
	NM_168338.2	NP_729485.1	5 - 6	947	60
GenBank mRNA	BT012467.1	-	4 - 5	743	60
	BT044219.1	-	5 - 6	1287	60
	U16139.1	-	4 - 5	792	60