Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Rab3-GEF
Entrez Gene ID: 32442
Gene Name: Rab3 GDP-GTP exchange factor
Gene Aliases: BEST:GH21304, CG5627, Dmel\CG5627, Dmel_CG5627, anon-EST:Posey246, rab3-GEF, rab3-gef
Chromosome Location: Chr.X: 15089682 - 15113762 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0030613

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001103513.3	NP_001096983.2	11 - 12	4058	76
	NM_001258746.2	NP_001245675.1	12 - 13	4076	76
	NM_001258747.1	NP_001245676.1	11 - 12	4034	76
	NM_001272630.1	NP_001259559.1	11 - 12	4034	76
	NM_001272631.1	NP_001259560.1	12 - 13	4067	76
	NM_001316635.1	NP_001303564.1	12 - 13	4067	76
	NM_001347809.1	NP_001334679.1	13 - 14	4118	76
	NM_001347810.1	NP_001334680.1	13 - 14	4118	76
	NM_001347811.1	NP_001334681.1	13 - 14	4118	76
	NM_132773.3	NP_573001.2	11 - 12	4034	76