Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: U2af50
Entrez Gene ID: 32602
Gene Name: U2 small nuclear riboprotein auxiliary factor 50
Gene Aliases: 9-21, CG9998, DU2AF50, DmU2AF[50], Dmel\CG9998, Dmel_CG9998, U2AF, U2AF 50, U2AF-50, U2AF50, U2af, dU2AF50, dU2AF[50], dUTAf[50], l(1)14Ca, l(1)14Cc, l(1)9-21
Chromosome Location: Chr.X: 16356696 - 16359039 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0005411

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001258779.1	NP_001245708.1	4 - 5	823	90
	NM_001258780.1	NP_001245709.1	4 - 5	850	90
	NM_001258781.2	NP_001245710.1	4 - 5	952	90
	NM_057543.4	NP_476891.1	4 - 5	1006	90
GenBank mRNA	AY069320.1	-	4 - 5	916	90
GenBank mRNA	L23404.1	-	4 - 5	917	90