Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: kn
Entrez Gene ID: 45318
Gene Name: knot
Gene Aliases: CG10197, Col, Col/Kn, DmCOE, Dmel\CG10197, Dmel_CG10197, Kn, Knot, coe, col, collier
Chromosome Location: Chr.2R: 14772650 - 14807061 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0001319

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001103847.2	NP_001097317.1	3 - 4	800	68
	NM_001274051.1	NP_001260980.1	2 - 3	469	68
	NM_080074.4	NP_524813.2	2 - 3	921	68
	NM_137146.3	NP_610990.2	2 - 3	921	68
	NM_166070.3	NP_725419.2	2 - 3	921	68
GenBank mRNA	BT150395.1	-	2 - 3	919	68