Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Pkn
Entrez Gene ID: 35950
Gene Name: Protein kinase N
Gene Aliases: 3-2, CG2049, CG2055, CG8, CT6637, CT6660, DPKN, Dmel\CG2049, Dmel_CG2049, Dpkn, PKC-related, PKN, PRK2, Pk45C, Pk?3, dPKN, l(2)06736, l(2)45Ca, pkn, v(2)rG232
Chromosome Location: Chr.2R: 9252310 - 9285097 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0020621

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001103767.2	NP_001097237.1	9 - 10	4659	89
	NM_001169624.1	NP_001163095.1	8 - 9	4008	89
	NM_001201966.2	NP_001188895.1	11 - 12	4603	89
	NM_001201967.1	NP_001188896.1	9 - 10	4218	89
	NM_001201968.3	NP_001188897.3	10 - 11	4123	89
	NM_001259291.2	NP_001246220.1	10 - 11	4064	89
	NM_001259292.2	NP_001246221.1	10 - 11	4195	89
	NM_001299312.1	NP_001286241.1	11 - 12	4715	89
	NM_001299313.1	NP_001286242.1	10 - 11	3785	89
	NM_001299314.1	NP_001286243.1	10 - 11	4124	89
	NM_001299315.1	NP_001286244.1	10 - 11	4118	89
	NM_176110.2	NP_788290.1	9 - 10	3854	89
	NM_176111.2	NP_788291.1	10 - 11	4505	89
	NM_176112.2	NP_788292.1	9 - 10	3976	89
	NM_176113.3	NP_788293.2	10 - 11	4627	89
	NM_176114.3	NP_788294.1	6 - 7	1724	89
GenBank mRNA	BT133091.1	-	10 - 11	4468	89
GenBank mRNA	BT133361.1	-	6 - 7	1733	89