Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: pug
Entrez Gene ID: 41279
Gene Name: pugilist
Gene Aliases: CG4067, Dmel\CG4067, Dmel_CG4067, MTHFD, Pug, bs14h02.y1, pugD
Chromosome Location: Chr.3R: 10696534 - 10703316 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0020385

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001014614.2	NP_001014614.1	2 - 3	227	86
	NM_001260099.1	NP_001247028.1	2 - 3	185	86
	NM_057906.4	NP_477254.1	1 - 2	165	86
	NM_169350.3	NP_731489.2	2 - 3	340	86
	NM_169351.2	NP_731490.1	2 - 3	111	86
GenBank mRNA	BT125950.1	-	2 - 3	210	86