Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: pum
Entrez Gene ID: 41094
Gene Name: pumilio
Gene Aliases: CG9755, CG9763, Dmel\CG9755, Dmel_CG9755, EP(3)0883, PKL, PUM, Pum, anon-WO0172774.19, bem, fs(3)02003, l(3)01688, ova, ovt, pkl
Chromosome Location: Chr.3R: 9066343 - 9237682 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0003165

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001260073.2	NP_001247002.1	3 - 4	1307	58
	NM_001275474.1	NP_001262403.1	9 - 10	3461	58
	NM_001275475.1	NP_001262404.1	2 - 3	1295	58
	NM_079561.4	NP_524285.2	9 - 10	3461	58
	NM_169258.3	NP_731314.1	9 - 10	3313	58
	NM_169259.3	NP_731315.1	9 - 10	3311	58
	NM_169260.4	NP_731316.2	7 - 8	1946	58
	NM_176427.3	NP_788604.1	3 - 4	1337	58
GenBank mRNA	BT001872.1	-	3 - 4	737	58