Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: mfas
Entrez Gene ID: 41455
Gene Name: midline fasciclin
Gene Aliases: 87A7-9/7, BEST:GH06752, CG3359, Dmel\CG3359, Dmel_CG3359, Mfas, anon-87Af, anon-EST:Liang-97, anon-WO0140519.174, clone 97, gh06752
Chromosome Location: Chr.3R: 11984862 - 11993315 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0260745

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001260137.1	NP_001247066.1	5 - 6	895	59
	NM_079600.3	NP_524324.2	6 - 7	1225	59
	NM_169444.1	NP_731661.1	6 - 7	913	59
	NM_176466.1	NP_788643.1	6 - 7	932	59
	NM_176467.1	NP_788644.1	6 - 7	910	59
	NM_176468.1	NP_788645.1	5 - 6	1309	59
	NM_176469.2	NP_788646.1	6 - 7	1030	59
	NM_176470.1	NP_788647.1	6 - 7	935	59
	NM_176471.1	NP_788648.1	6 - 7	1108	59
	NM_176472.1	NP_788649.1	6 - 7	1130	59
	NM_176473.1	NP_788650.1	7 - 8	1004	59
	NM_176474.1	NP_788651.1	7 - 8	982	59
	NM_176475.1	NP_788652.1	6 - 7	1381	59
	NM_176476.1	NP_788653.1	7 - 8	985	59
	NM_176477.1	NP_788654.1	7 - 8	1007	59
	NM_176478.1	NP_788655.1	7 - 8	1180	59
GenBank mRNA	AY069094.1	-	6 - 7	895	59
	BT120190.1	-	5 - 6	895	59
	BT125878.1	-	7 - 8	981	59
	BT133296.1	-	7 - 8	1159	59