Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Nelf-A
Entrez Gene ID: 42520
Gene Name: Negative elongation factor A
Gene Aliases: BcDNA:SD07139, CG5874, Dmel\CG5874, Dmel_CG5874, NELF, NELF-A
Chromosome Location: Chr.3R: 21223704 - 21229512 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0038872

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001275875.1	NP_001262804.1	5 - 6	3637	56
	NM_001300509.1	NP_001287438.1	5 - 6	3634	56
	NM_142701.2	NP_650958.2	5 - 6	3637	56
GenBank mRNA	BT150479.1	-	5 - 6	3654	56