Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Pak
Entrez Gene ID: 44039
Gene Name: p21-activated kinase
Gene Aliases: CG10295, D-Pak, DPAK, DPak, DPak1, DmPAK1, Dmel\CG10295, Dmel_CG10295, Dpak, Dpak1, PAK, PAK-1, PAK1, PAK2, PaK, Pak1, dPAK, dPAK1, dPak, dpak, dpak1, l(3)psg4, p65[PAK], pak, pak1, psg4
Chromosome Location: Chr.3R: 6350896 - 6361614 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0267698

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001144541.2	NP_001138013.2	6 - 7	2790	80
	NM_001275380.2	NP_001262309.1	6 - 7	2084	80
	NM_001275381.2	NP_001262310.1	6 - 7	2497	80
	NM_001275382.1	NP_001262311.1	6 - 7	2751	80
	NM_001275383.1	NP_001262312.1	6 - 7	2492	80
	NM_001275384.1	NP_001262313.1	6 - 7	2382	80
	NM_079942.4	NP_524681.1	6 - 7	2089	80
	NM_169136.3	NP_731073.1	6 - 7	2382	80
	NM_169137.3	NP_731074.1	6 - 7	1985	80
GenBank mRNA	BT010291.1	-	6 - 7	2350	80