Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: spz
Entrez Gene ID: 43256
Gene Name: spatzle
Gene Aliases: CG6134, CT19282, Dmel\CG6134, Dmel_CG6134, SPZ, Spz, Spz-1, Spz1, mel(3)7
Chromosome Location: Chr.3R: 27064990 - 27070070 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0003495

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001104472.2	NP_001097942.1	5 - 6	1558	57
	NM_001144644.2	NP_001138116.1	4 - 5	1498	57
	NM_079802.3	NP_524526.1	4 - 5	1666	57
	NM_170309.2	NP_733188.1	3 - 4	1369	57
	NM_170312.2	NP_733191.1	3 - 4	1588	57
	NM_170313.2	NP_733192.1	4 - 5	1420	57
	NM_170315.2	NP_733194.1	4 - 5	1447	57
	NM_170316.2	NP_733195.1	4 - 5	1507	57
GenBank mRNA	AF237968.1	-	4 - 5	586	57
	AF237969.1	-	4 - 5	805	57
	EF155533.1	-	4 - 5	637	57