Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: SkpA
Entrez Gene ID: 31016
Gene Name: SKP1-related A
Gene Aliases: CG16983, Dmel\CG16983, Dmel_CG16983, EG:115C2.4, SKP1, SKPA, Skp, Skp1, anon-WO03040301.234, anon-WO03040301.236, anon-WO03040301.238, dSKP1, dSkip-1, dSkp-1, dSkpA, dskpA, l(1)G0037, l(1)G0058, l(1)G0109, l(1)G0389, skpA, spkA
Chromosome Location: Chr.X: 656114 - 657899 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0025637

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001038729.3	NP_001033818.1	1 - 1	851	60
	NM_001297826.1	NP_001284755.1	1 - 1	133	60
	NM_058042.5	NP_477390.1	2 - 2	394	60
	NM_166856.3	NP_726690.1	2 - 2	242	60
	NM_166857.3	NP_726691.1	2 - 2	357	60
	NM_166858.3	NP_726692.1	2 - 2	779	60
	NM_166859.3	NP_726693.1	2 - 2	176	60
	NM_166860.3	NP_726694.1	2 - 2	220	60
	NM_166861.3	NP_726695.1	2 - 2	152	60
GenBank mRNA	AY069297.1	-	1 - 1	813	60
	BT099582.1	-	2 - 2	180	60
	BT099610.1	-	2 - 2	337	60
	KX532038.1	-	1 - 1	97	60