Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Act5C
Entrez Gene ID: 31521
Gene Name: Actin 5C
Gene Aliases: A, A4V404_DROME, ACT, ACT1_DROME, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, ArpA, BAP47, Bap47, CG4027, Dmel\CG4027, Dmel_CG4027, F-actin, G-actin, M32055, T11, act, act 5C, act5C, actin, actin5C, anon-EST:fe2D2, beta-actin, beta-actin/Bap47, chrX:5748184..5748304, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486
Chromosome Location: Chr.X: 5900861 - 5905399 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0000042

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001014725.2	NP_001014725.1	2 - 2	1514	125
	NM_001014726.2	NP_001014726.1	2 - 2	1514	125
	NM_001297986.1	NP_001284915.1	2 - 2	1514	125
	NM_078497.4	NP_511052.1	2 - 2	1476	125
	NM_167053.2	NP_727048.1	2 - 2	1514	125
GenBank mRNA	AY089562.1	-	2 - 2	1508	125