The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001081838.1 | NP_001075307.1 | 1 - 1 | 67 | 108 |
| XM_023655002.1 | XP_023510770.1 | - | 205 | 108 | |
| GenBank mRNA | AF035774.1 | - | 1 - 1 | 67 | 108 |