The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_018571.5 | NP_061041.2 | 11 - 11 | 1457 | 66 |
| XM_005246669.1 | XP_005246726.1 | 11 - 11 | 1205 | 66 | |
| XM_017004438.1 | XP_016859927.1 | 10 - 10 | 1292 | 66 | |
| GenBank mRNA | AB038950.1 | - | 11 - 11 | 1333 | 66 |
| AF116618.2 | - | 7 - 7 | 894 | 66 | |
| AK024720.1 | - | 7 - 7 | 811 | 66 | |
| AK027637.1 | - | 11 - 11 | 1457 | 66 | |
| AK290457.1 | - | 12 - 12 | 1321 | 66 | |
| AY093697.1 | - | 11 - 11 | 1345 | 66 | |
| BC008302.1 | - | 11 - 11 | 1359 | 66 | |