Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001029851.2	NP_001025022.1	19 - 19	2487	82
	NM_001029852.2	NP_001025023.1	21 - 21	2613	82
	NM_001029853.2	NP_001025024.1	21 - 21	2718	82
	NM_001029854.2	NP_001025025.1	21 - 21	2637	82
	NM_003719.3	NP_003710.1	22 - 22	2778	82
	XM_005248621.4	XP_005248678.1	22 - 22	3004	82
	XM_005248623.4	XP_005248680.1	22 - 22	2629	82
	XM_005248624.4	XP_005248681.1	22 - 22	2573	82
	XM_006714725.3	XP_006714788.1	23 - 23	3071	82
	XM_006714726.3	XP_006714789.1	23 - 23	3068	82
	XM_011543699.2	XP_011542001.1	22 - 22	2943	82
	XM_011543700.2	XP_011542002.1	20 - 20	2781	82
	XM_011543701.2	XP_011542003.1	23 - 23	2584	82
	XM_011543702.2	XP_011542004.1	22 - 22	2415	82
	XM_011543703.2	XP_011542005.1	22 - 22	2433	82
	XM_011543704.2	XP_011542006.1	20 - 20	2236	82
	XM_017010003.1	XP_016865492.1	21 - 21	2876	82
	XM_017010004.1	XP_016865493.1	22 - 22	2412	82
	XM_017010005.1	XP_016865494.1	19 - 19	2714	82
	XM_017010006.1	XP_016865495.1	23 - 23	2851	82
	XM_017010007.1	XP_016865496.1	21 - 21	2489	82
	XM_017010008.1	XP_016865497.1	19 - 19	2328	82
GenBank mRNA	AF079529.2	-	22 - 22		82
	AK002074.1	-	11 - 11		82
	AK023913.1	-	17 - 17		82
	AY129948.1	-	21 - 21		82
	AY129949.1	-	19 - 19		82
	BC035144.1	-	9 - 9		82
	BC047627.1	-	5 - 5		82
	BE169437.1	-	1 - 1	67	82