The assay primers and probes lie within a single exon. This assay will detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001099658.1 | NP_001093128.1 | 3 - 3 | 2630 | 107 |
| NM_001099660.1 | NP_001093130.1 | 4 - 4 | 2737 | 107 | |
| NM_018334.4 | NP_060804.3 | 2 - 2 | 2548 | 107 | |
| GenBank mRNA | AB060967.1 | - | 1 - 1 | 1997 | 107 |
| AF134481.1 | - | 1 - 1 | 2028 | 107 | |
| AK292556.1 | - | 2 - 2 | 2137 | 107 | |
| AL442092.1 | - | 1 - 1 | 1953 | 107 | |
| AY358280.1 | - | 3 - 3 | 2170 | 107 | |
| BC035133.2 | - | 2 - 2 | 2427 | 107 | |