The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001035247.2 | NP_001030324.2 | 6 - 6 | 599 | 106 |
| NM_005659.6 | NP_005650.2 | 6 - 6 | 599 | 106 | |
| XM_011530361.2 | XP_011528663.1 | 6 - 6 | 725 | 106 | |
| GenBank mRNA | AF141201.1 | - | 5 - 5 | 469 | 106 |
| AJ239058.1 | - | 5 - 5 | 469 | 106 | |
| AK225877.1 | - | 6 - 6 | 556 | 106 | |
| AK304734.1 | - | 6 - 6 | 569 | 106 | |
| AK315998.1 | - | 6 - 6 | 550 | 106 | |
| AY101594.1 | - | 6 - 6 | 526 | 106 | |
| BC001049.2 | - | 6 - 6 | 546 | 106 | |
| BC005087.1 | - | 6 - 6 | 547 | 106 | |
| BM761958.1 | - | 5 - 5 | 538 | 106 | |
| BX648483.1 | - | 6 - 6 | 531 | 106 | |
| CR456607.1 | - | 6 - 6 | 483 | 106 | |