The assay primers and probes lie within a single exon. This assay will detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001301228.1 | NP_001288157.1 | 5 - 5 | 1713 | 169 |
| NM_001301229.1 | NP_001288158.1 | 5 - 5 | 1604 | 169 | |
| NM_001301231.1 | NP_001288160.1 | 5 - 5 | 1643 | 169 | |
| NM_005342.3 | NP_005333.2 | 5 - 5 | 1608 | 169 | |
| GenBank mRNA | BX537505.1 | - | 3 - 3 | 3002 | 169 |
| Y10043.1 | - | 5 - 5 | 1584 | 169 | |