The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001173984.2 | NP_001167455.1 | 15 - 15 | 1838 | 123 |
| NM_013263.4 | NP_037395.2 | 15 - 15 | 1835 | 123 | |
| XM_011523046.2 | XP_011521348.1 | 15 - 15 | 1924 | 123 | |
| XM_011523047.2 | XP_011521349.1 | 15 - 15 | 1921 | 123 | |
| XM_011523048.2 | XP_011521350.1 | 14 - 14 | 1816 | 123 | |
| XM_011523049.2 | XP_011521351.1 | 14 - 14 | 1813 | 123 | |
| XM_011523050.2 | XP_011521352.1 | 11 - 11 | 1944 | 123 | |
| XM_017023179.1 | XP_016878668.1 | 14 - 14 | 1630 | 123 | |
| XM_017023180.1 | XP_016878669.1 | 14 - 14 | 1627 | 123 | |
| XM_017023181.1 | XP_016878670.1 | 11 - 11 | 1958 | 123 | |
| GenBank mRNA | AF152604.1 | - | 15 - 15 | 1728 | 123 |
| AF213969.1 | - | 15 - 15 | 1683 | 123 | |
| AJ271881.1 | - | 15 - 15 | 1793 | 123 | |
| AK027308.1 | - | 8 - 8 | 806 | 123 | |
| AK074613.1 | - | 9 - 9 | 972 | 123 | |
| BC001611.1 | - | 11 - 11 | 1099 | 123 | |
| BC050728.1 | - | 16 - 16 | 1742 | 123 | |
| BC094706.1 | - | 16 - 16 | 1712 | 123 | |