The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001114120.2 | NP_001107592.1 | 2 - 2 | 294 | 102 |
| NM_017779.5 | NP_060249.2 | 2 - 2 | 294 | 102 | |
| GenBank mRNA | AB281187.1 | - | 2 - 2 | 150 | 102 |
| AB281274.1 | - | 2 - 2 | 150 | 102 | |
| AJ278112.1 | - | 2 - 2 | 150 | 102 | |
| AK000361.1 | - | 2 - 2 | 155 | 102 | |
| AK225735.1 | - | 2 - 2 | 140 | 102 | |
| BC003511.1 | - | 2 - 2 | 143 | 102 | |
| BC065304.1 | - | 2 - 2 | 97 | 102 | |
| DB147703.1 | - | 2 - 2 | 294 | 102 | |