Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: BRF1
Entrez Gene ID: 2972
Gene Name: BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit
Gene Aliases: BRF, BRF-1, CFDS, GTF3B, HEL-S-76p, TAF3B2, TAF3C, TAFIII90, TF3B90, TFIIIB90, hBRF
Chromosome Location: Chr.14: 105209286 - 105315577 on Build GRCh38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001242786.1	NP_001229715.1	8 - 9	985	73
	NM_001242787.1	NP_001229716.1	8 - 9	985	73
	NM_001242788.1	NP_001229717.1	7 - 8	1195	73
	NM_001242789.1	NP_001229718.1	3 - 4	475	73
	NM_001519.3	NP_001510.2	8 - 9	1276	73
	NM_145685.2	NP_663718.1	4 - 5	1184	73
	XM_005267561.3	XP_005267618.1	8 - 9	1298	73
	XM_005267563.3	XP_005267620.1	4 - 5	1988	73
	XM_006720123.2	XP_006720186.1	3 - 4	1416	73
	XM_011536672.2	XP_011534974.1	8 - 9	1298	73
	XM_011536673.1	XP_011534975.1	7 - 8	1148	73
	XM_011536674.1	XP_011534976.1	4 - 5	1566	73
GenBank mRNA	AJ297407.1	-	4 - 5	1192	73
	AK096471.1	-	3 - 4	475	73
	AK294899.1	-	8 - 9	985	73
	AK295579.1	-	8 - 9	898	73
	BC071637.1	-	3 - 4	340	73
	BC086856.1	-	7 - 8	928	73
	BC103861.2	-	4 - 5	492	73
	BC133655.1	-	4 - 5	492	73
	U28838.1	-	8 - 9	1279	73
	U75276.1	-	8 - 9	980	73