The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_020139.3 | NP_064524.3 | 7 - 7 | 589 | 182 |
| XM_006714274.3 | XP_006714337.1 | 8 - 8 | 626 | 182 | |
| XM_011532127.2 | XP_011530429.1 | 8 - 8 | 668 | 182 | |
| XM_011532128.2 | XP_011530430.1 | 7 - 7 | 626 | 182 | |
| XM_017008461.1 | XP_016863950.1 | 8 - 8 | 665 | 182 | |
| XM_017008462.1 | XP_016863951.1 | 7 - 7 | 623 | 182 | |
| GenBank mRNA | AF164790.1 | - | 7 - 7 | 553 | 182 |
| AK023323.1 | - | 6 - 6 | 183 | ||
| AK128463.1 | - | 7 - 7 | 2304 | 182 | |
| AK300626.1 | - | 7 - 7 | 434 | 182 | |
| BC001953.1 | - | 7 - 7 | 582 | 182 | |
| BC037277.2 | - | 6 - 6 | 1568 | 182 | |
| BC095414.1 | - | 7 - 7 | 557 | 182 | |